Reader in Insect Biochemistry

Liverpool School of Tropical Medicine, UK

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Website: http://www.lstmed.ac.uk/about/people/dr-mark-paine

Mark Paine is Reader in Insect Biochemistry and works on:

1. Research on molecular mechanisms of insecticide resistance and to translate this knowledge into practical solutions for tropical diseases. Mosquitoes and sandflies are the major focus, being responsible for the transmission of malaria, arboviruses and leishmaniasis.
2. The identification of proteins associated with resistance and the investigation on how the detoxification process works to develop diagnostics for predicting and monitoring resistance.
3. The development of diagnostics for detection and monitoring of insecticides (Insecticide Quantification Kits) in connection with the Innovative Vector Control Consortium (IVCC) for the production of improved insecticides, formulations and decision support tools to reduce disease transmission.

 Publications:

• Stevenson BJ , Pignatelli P , Nikou D , Paine MJI (2012) Pinpointing P450s Associated with Pyrethroid Metabolism in the Dengue Vector, Aedes aegypti: Developing New Tools to Combat Insecticide Resistance. PLoS Negl Trop Dis 6(3).
• Mitchell, S.N., Stevenson, B., Mueller, P., Wilding, C.S., Egyir-Yawson, A., Field, S., Hemingway, J., Paine,M.J.I., Ranson, H., and Donnelly, M.J. (2012) Identification and Validation of a gene causing cross-resistance between insecticide classes in Anopheles gambiae in Ghana. Proc Natl Acad Sci USA.
• David JP, Ismail HM, Chandor-Proust A, Paine MJ (2013) Role of cytochrome P450s in insecticide resistance: impact on the control of mosquito-borne diseases and use of insecticides on Earth. Philos Trans R Soc Lond B Biol Sci 368: 20120429.
• Riveron, J.M., Irving, H., Ndula, M., Barnes, K.G., Ibrahim, S., Cuamba, N., Paine, M.J.I. & Wondji, C. (2013) Directionally selected cytochrome P450 alleles are driving the spread of pyrethroid resistance in the major malaria vector Anopheles funestus. Proc Natl Acad Sci U S A 110: 252-257
• Chandor-Proust, A., Bibby, J., Régent-Kloeckner, M., Roux, J., Guitard, E., Poupardin, R., Riaz, M.A., Paine, M.J.I., Dauphin-Villemant, C., Reynaud, S., and David, JP (2013) The central role of mosquito cytochrome P450 CYP6Zs in the detoxification of pyrethroid revealed by functional expression and structural modelling. Biochem J. 2013 Oct 1;455(1):75-85.
• Ismail, H., O’Neill, P., Hong, D., Finn, R.D., Henderson, C.J. Wright, A.T., Cravatt, B.F., Hemingway, J., and Paine, M.J.I. (2013) Pyrethroid Activity-Based Probes for Profiling Cytochrome P450 Activities Associated with Insecticide Interactions Proc National Acad Sci. USA. Nov 18th early edition.
• Russell, T.L., Morgan, J.C., Ismail,H., Kaur, H., Eggelt, T., Oladepo, F., Amon, J., Hemingway, J., Iata, H., and Paine, M.J.I (2014) Evaluating the feasibility of using insecticide quantification kits (IQK) for estimating insecticidal levels for IRS in Vanuatu. Malaria J. 13, 178
• Riga, M., Tsakireli, D., Ilias, A., Morou, E., Myridakis, A., Stephanou, E.G., Nauen, R., Dermauw, W., Van Leeuwen, T., Paine, M., and Vontas, J. (2014) Insect biochemistry and molecular biology 46, 43-53.
• Mitchell, S.N., Rigden, D.J., Dowd, A.J., Lu, F., Wilding, C.S., Weetman, D., Dadzie, S., Jenkins, A.M., Regna, K., Boko, P., Djogbenou, L., Muskavitch, M.A., Ranson, H., Paine, M.J., Mayans, O., and Donnelly, M.J. (2014) Metabolic and Target-Site Mechanisms Combine to Confer Strong DDT Resistance in Anopheles gambiae. PloS one 9, e92662
• Edi, C.V., Djogbenou, L., Jenkins, A.M., Regna, K., Muskavitch, M.A., Poupardin, R., Jones, C.M., Essandoh, J., Ketoh, G.K., Paine, M.J., Koudou, B.G., Donnelly, M.J., Ranson, H., and Weetman, D. (2014) CYP6 P450 Enzymes and ACE-1 Duplication Produce Extreme and Multiple Insecticide Resistance in the Malaria Mosquito Anopheles gambiae. PLoS genetics 10, e1004236
• Coleman, M.; Foster, G.M.; Deb, R.; Singh, R P.; Ismail, H.M.; Shivam, P.; Ghosh, A.K.; Dunkley, S.; Kumar, V.; Coleman, M., Paine, M.J.I., Das, P. (2015) DDT-based indoor residual spraying suboptimal for visceral leishmaniasis elimination in India. Proceedings of the National Academy of Sciences 112, 8573-857.
• Ismail, H.M.; Kumar, V.; Singh, R.P.; Williams, C.; Shivam, P.; Ghosh, A.; Deb, R.; Foster, G.M.; Hemingway, J.; Coleman, M.; Coleman, M.; Das, P.; Paine, M.J., Development of a Simple Dipstick Assay for Operational Monitoring of DDT. (2016) PLoS NTD.